Thermo fisher scientific has launched the applied biosystems quantstudio 3 and quantstudio 5, realtime pcr systems that fully integrate with cloud computing technology. Lti, carlsbad, ca, which is representative of other available highthroughput pcrbased genotyping platforms. We also recommend you to check the files before installation. The protection provided by the equipment may be impaired if the instrument is operated outside the environment and use specifications, the user. Students learn to efficiently choose and solve partial products that enable better mental multiplication with multidigit numbers. For safety and biohazard guidelines, refer to the safety appendix in the quantstudio 3d digital pcr system user guide page 2 2. L of the appropriate calibration solution into each of the eight reservoirs in the array card. A brief summary of the content of the site is below.
Easily run up to 4 quantstudio 12k flex openarray plates for any application on the quantstudio 12k flex instrument in standalone mode. Openarray technology overview thermo fisher scientific. Quantstudio and flex software the quantstudio software allows the user to open and analyze experiments generated with the viia7 system, quantstudio 6 flex, and 7 flex realtime pcr systems. Applied biosystems quantstudio 3d quick reference pdf download. The quantstudio 3 realtime pcr system is compact in form, factory calibrated and is delivered in user friendly packaging to enable fast and simple installation for immediate use. When equipped with the openarray block, a single 9700 thermal cycler and the quantstudio 12k flex applied biosystems openarray accufill system, the quantstudio 12k flex system can produce up to 110,000 data points or more in an eighthour day. Applied biosystems quantstudio 6 flex realtime pcr system.
The contents of the download are original and were not modified in any way. Chapter 3 calibrating openarray plate sample blocks. Start a 12,000 data point experiment typically in 20. Quantstudio 6 and 7 flex realtime pcr systems maintenance and administration guide 11 about this guide purpose this user guide provides provides reference information for the quantstudio 6 and 7 flex realtime pcr systems and describes how to prepare, maintain, and. We are using thermofishers quantstudio 3 and 5 instruments. Equipment supported by the core includes a abi quantstudio 12k flex with 96well, 384well, and open array options and an abi 7900ht with 96well and 384well plate options. Appendix d power on or off, store, and move move the quantstudio 6 or 7 flex system prepare the 1. Quantstudio 12k flex realtime pcr system thermo fisher. The quantstudio 6 and 7 pro realtime pcr systems will still improve your workflow. Start a 12,000 data point experiment in typically less than 20 minutes using automated sample produce up to 110,000 data points or more in loading with the quantstudio 12k flex accufill system figure 2. Quantstudio 6 and 7 flex realtime pcr system software experiments appendixes common information such as ordering information, additional documentation, and. The software measures the fluorescence across the image. The interactive touchscreen provides a modern usability experience and the simplified instrument softwarepreoptimized protocol templates allow users of any.
For safety and biohazard guidelines, refer to the safety appendix in the quantstudio 3d digital pcr system user guide. After looking over beginningoftheyear assessments, a new 4th grade teacher was concerned that half of her students were still unsure of the open array model. Quantstudio 12k flex realtime pcr system from thermo fisher. The download version of quantstudio design analysis software is 1. Quantstudio design analysis software free download windows.
Load the reaction plate or array card into the instrument. When equipped with the open array block and the quantstudio 12kflex openarray accufill system,the quantstudio 12kflex system can produce up to 110,000 data points or more in an 8hour. The surface retention system holds the sample in place eliminating the need for. Quantstudio 3d digital pcr instrument dual flat block geneamp pcr system 9700 quantstudio 3d digital pcr master mix quantstudio 3d digital pcr 20k chip quantstudio 3d digital pcr chip loader catalog number 4482593 and 4481097 publication number man0007720 revision a. Guide to running kasp genotyping reactions on the applied. Except for importants, the safety alert words in user documentation appear with an open triangle figure that contains a hazard symbol. Quantstudio 6 and 7 flex realtime pcr system software. Quantstudio 3d digital pcr system user guide garvan institute of. Quantstudio 6 and 7 flex realtime pcr systems maintenance and administration guide 11 about this guide purpose this user guide provides provides reference information for the quantstudio 6 and 7 flex realtime pcr systems and describes how to prepare, maintain, and troubleshoot the systems. The nanodrop 8000 utilizes the same patented sample retention technology employed on all nanodrop instruments.
Purpose the quantstudio 12k flex realtime pcr system multiwell plates and array card experiments user guide binder functions as both a tutorial and as a guide for performing your own experiments using the 384well, 96well 01. The information in this publication is provided for reference only. Quantstudio 12k flex realtime pcr system from thermo. Quantstudio 6 and 7 pro realtime pcr systems by thermo. Quantstudio and flex software free version download for pc. Do not open heated lid to stop the run from a final hold. The systems are designed for low to midthroughput laboratories conducting realtime pcr experiments, and are the first to connect to the thermo fisher cloud computing platform, allowing geographically. It also enables you to set up experiments, send experiments to the instrument, collect data, and analyze the collected data all in an. This unique technology minimizes reagent use and helps greatly decrease the price per data point, so more samples can be run within the same budget. The quantstudio dx realtime pcr instrument is for distribution and use in the united states and specific european countries only. With single database management of up to 8 instruments per computer, the biofire filmarray 2. Using this product in a manner not specified in the user documentation may result in. The applied biosystems quantstudio 12k flex realtime pcr system openarray.
The quantstudio dx realtime pcr instrument with quantstudio dx software is intended to perform fluorescencebased pcr to provide detection of nucleic acid sequences in humanderived specimens in ivd labeled tests. Refer to the quantstudio 6 and 7 flex realtime pcr systems maintenance and administration guide for detailed instructions on creating the array cards required to calibrate the quantstudio 7 flex system. Comparison of digital pcr platforms and seminested qpcr. See fill an array card quantstudio 7 flex system only on page 8 for the general procedure for filling and sealing array cards. If you plan to store the quantstudio 6 or 7 flex realtime pcr system for more than a week or you plan to move it, load an unused plate or array card into the quantstudio. Applied biosystems quantstudio 3 realtime pcr system, 96. Applied biosystems quantstudio 3 realtime pcr system, 96well, 0. For this study, the threshold was user defined at 4000. User manual of the quanta lb6m series layer 2, 3, and ipv6 plus qos managed switch. Table 1 lists the commonly used models and some of their important characteristics, some of the models are also discussed in detail. Quantstudio 6 and 7 flex realtime pcr system software getting started guide for melt curve experiments designing, running, and analyzing a melt curve experiment. This user guide contains the following information.
Pricing does not include the required thermpfisher open array kits. Review the concentration of your dna samples, and prepare load and seal the chips. Applied biosystems quantstudio 12k flex real time pcr. Firsttime users of the quantstudio 6 and 7 flex realtime pcr system. Please check with your local sales rep for pricing or talk to us to help you make that contact. The protection provided by the equipment may be impaired if the instrument is operated outside the envi ronment and use specifications, the user. If not, perform the calibration again using the correct normalization plate or array card. Chapter 2, calibrating multiwell plate and array card sample blocks describes how to maintain a quantstudio 12k flex system with a 96384well plate or array card sample block, including calibration and performance verification. In the setup menu, the first stage is to define the experiment properties. If instrument does not do any background correction in this case, how the data should be processed for further analysis. Thermo fisher scientifics applied biosystems quantstudio.
Pcr machines are a musthave in any life science research lab today. Thermo fisher scientifics applied biosystems quantstudio 3. Applied biosystems quantstudio 3d quick reference pdf. Taqman array plates with rfid will also work to help reduce manual errors in loading your assay plate settings and loading the plate setup. The global market of these devices has substantially grown in the past three decades with a large number of models with various characteristics. Highthroughput realtime pcrbased genotyping without dna. This realtime pcr system is compatible with the full range of taqman assays, and interchangeable quantstudio 12k flex 96well, fast 96well and 384well blocks, as well as open array plates, letting you go from 1 to 12000 data points within the same run. Quant studio 6 software guide purdue biological sciences. Applied biosystems quantstudio 12k flex realtime pcr system maintenance and administration user. Jan 23, 2012 quantstudio 12k flex openarray accufill system, the quantstudio 12k flex system can reduce startup and handson time. Openarray plate quick reference about the background calibration data during the background calibration, the quantstudio 12k flex software captures a series of images of the black side of the openarray calibration plaque using each instrument filter.
Janice october 10, 20 february, 2016 array model, models, multiplication strings, questioning. About this guide purpose quantstudio 3 and 5 realtime pcr systems installation, use, and maintenance guide 9. In case of no passive reference dyerox in a qpcr experiment, how instrument handles background correction. Quantstudio 7 flex realtime pcr instrument, 96well from. Raw data analysis was performed with the specific software for each of the three platforms, i. Taqman openarray genotyping troubleshooting guide pub. For the ultimate in productivity, consider the quantstudio 12k flex system, which also accommodates taqman openarray plates. The software also enables the user to set up experiments, send experiments to the instrument, control the thermal cycling process, collect data and analyze. Comparison of digital pcr platforms and seminested qpcr as a.
Oct 19, 2012 genotyping of 12,000 targets in one run was conducted through the use of the taqman sampletosnp kit and the quantstudio 12k realtime pcr system life technologies inc. Thank you for downloading quantstudio design analysis software from our software library. Page 1 quantstudio 6 and 7 flex realtime pcr systems user guide maintenance and administration for use with quantstudio real. For the quantstudio quantifications, automated threshold setting proved highly unreliable for the smallest input samples, and the analysis software does not enable direct comparison of the samples, thereby creating user bias when manually setting one uniform threshold. Multiwell plates and array card experiments user guide part no.
Quantstudio 7 flex realtime pcr instrument, 96well from thermo fisher scientific description the applied biosystems quantstudio 7 flex realtime pcr system advances your research further by enabling a broad range of realtime pcrbased applications through its multiplexing capabilities and interchangeable block formats. You can still maximize uptime with smart help and smart remote support features to streamline technical support help. Use the buttons or the horizontal navigation above to learn more about the openstudio suite of applications. Realtime pcr openarray thermo fisher scientific us. User manual of the quanta lb6m series layer 2, 3, and ipv6. Life technologies corporation skupart number product namedescription list price nys discount nys net price amafd evos fl auto, no objno cube each 57,500. Open usb port the instrument can only be connected via the usb. Confirm that you loaded an normalization plate or array card into the quantstudio 12k flex instrument. Openarray technology helps streamline realtime pcr studies that use large numbers of samples, assays, or both, because a single quantstudio 12k flex openarray plate is equivalent to running eight traditional. Applied biosystems quantstudio 12k flex realtime pcr system.
How to handle no passive reference case in qpcr experiment. Flex realtime pcr systems and describes how to prepare, maintain, and troubleshoot the systems. Openarray technology is an affordable realtime pcrbased solution for highthroughput gene expression analysis, genotyping, microrna mirna analysis, and digital pcr applications. Quantstudio 12k flex realtime pcr system with openarray block with accufill system taqman openarray genotyping plates taqman genotyper software copycaller software alleletyper software catalog numbers 4475395, 90, 4486060 publication number man0009612 revision c. Nov 15, 2012 this month we are featuring multiplication.
Applied biosystems quantstudio 6 flex user manual pdf. Lti, carlsbad, ca, which is representative of other available high. This user guide provides stepbystep instructions for preparing and loading samples. On the front screen, in the set up box, click on experiment setup. Use lintfree wipe with isopropanol or ethanol to remove immersion fluid from lid prevent aqueous condensation quantstudio3d digital pcr chips. The quantstudio 6 flex system can also be upgraded to a quantstudio 7 flex system at a later date, as your needs change.
Download quantstudio design analysis software for free. Quantitative pcr, genotyping and quantstudio open array the core provides equipment and technical assistance for performance of quantitative pcr realtime pcr. The quantstudio design and analysis desktop software allows the user to open, run, and analyze experiments generated with quantstudio 1 realtime pcr system, quantstudio 3 realtime pcr system, and quantstudio 5 realtime pcr system. This dreambox lesson ensures students connect partial products to a concrete area context on the open array. User guide quantstudio 3d digital pcr system for use with. The quantstudio 12k flex realtime pcr system includes easytochange blocks that can accommodate openarray plates, taqman array cards, 384well, 96well, and fast 96well plates allowing you to select the format that is right for your project whether its now or in the future, for maximum flexibility and consistency in your research. Download free quantstudio design analysis software 1. The download was scanned for viruses by our system. Quantstudio design analysis software free download. Pharmacogenomics experiments application guide pub. The systems are designed for low to midthroughput laboratories conducting realtime pcr experiments, and are the first to connect to the thermo fisher cloud computing platform, allowing geographically dispersed researchers. Cost per array is for laboratory processing, open array instrument runs, and data export.
Genotyping of 12,000 targets in one run was conducted through the use of the taqman sampletosnp kit and the quantstudio 12k realtime pcr system life technologies inc. The instrument is controlled by pc based software, and the data is logged in. Integrated tracking software help to collect and monitor the abundance of information as. Quantstudio 12k flex realtime pcr system maintenance and. Experiments user guide functions as both a tutorial. This section provides installation instructions, introductory tutorials, and information to get you up and running. With heated lid closed, allow thermal cycler to sit 5 min remove chips note. Applied biosystems quantstudio 6 flex user manual pdf download. Page 1 quick reference quantstudio 3d digital pcr system catalog number 4482593 and 4481097 pub.
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